种质与环境对二年生甘草群体主要生物活性成分的影响(3)
YU Fu，lai1，3， WANG Wen，quan2，3，4*， HOU Jun，ling3，4*， ZHOU Xiang，zhen3， HAN Ya，nan3， WANG Dan1
(1. Tropical Crops Genetic Resources Institute， Chinese Academy of Tropical Agricultural Sciences，Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China， Danzhou 571737， China；
2. Institute of Medicinal Plant Development， Chinese Academy of Medical
Sciences & Peking Union Medical College， Beijing 100193， China；
3. School of Chinese Pharmacy， Beijing University of Chinese Medicine， Beijing 100102， China；
4. Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs，Ministry of Education， Beijing 100102， China)
Objective： This study aimed at analyzing the effect of genotype (G)， environment (E) and their interactions (G×E) on the major bioactive components of 2，year licorice (Glycyrrhiza uralensis) population， in order to provide a theoretical basis for the licorice breeding with high content of bioactive components and quality improvement.
Method： Four genotype licorice populations were transplanted under four different environments by using complete randomized block design with three replicates， and four major bioactive components， including glycyrrhizin (GL)， total saponins (TS)， liquiritin (LQ) and total flavonoids (TF) were determined by UV and by HPLC.
Result： The major bioactive components of licorice were influenced by genotype and environment， and the genotype had more effect on all of the bioactive components. The contents of GL and LQ were codetermined by genotype and environment factors.
Conclusion： There exist different selective effects on different growth region for quality breeding in cultivated population of licorice.
[Key words]licorice (Glycyrrhiza uralensis)； bioactive components； genotype； environment； quality breeding
doi：10.4268/cjcmm20131004, http://www.100md.com(于福来 王文全 侯俊玲 周香珍 韩亚男 王丹)
(1. Tropical Crops Genetic Resources Institute， Chinese Academy of Tropical Agricultural Sciences，Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China， Danzhou 571737， China；
2. Institute of Medicinal Plant Development， Chinese Academy of Medical
Sciences & Peking Union Medical College， Beijing 100193， China；
3. School of Chinese Pharmacy， Beijing University of Chinese Medicine， Beijing 100102， China；
4. Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs，Ministry of Education， Beijing 100102， China)
Objective： This study aimed at analyzing the effect of genotype (G)， environment (E) and their interactions (G×E) on the major bioactive components of 2，year licorice (Glycyrrhiza uralensis) population， in order to provide a theoretical basis for the licorice breeding with high content of bioactive components and quality improvement.
Method： Four genotype licorice populations were transplanted under four different environments by using complete randomized block design with three replicates， and four major bioactive components， including glycyrrhizin (GL)， total saponins (TS)， liquiritin (LQ) and total flavonoids (TF) were determined by UV and by HPLC.
Result： The major bioactive components of licorice were influenced by genotype and environment， and the genotype had more effect on all of the bioactive components. The contents of GL and LQ were codetermined by genotype and environment factors.
Conclusion： There exist different selective effects on different growth region for quality breeding in cultivated population of licorice.
[Key words]licorice (Glycyrrhiza uralensis)； bioactive components； genotype； environment； quality breeding
doi：10.4268/cjcmm20131004, http://www.100md.com(于福来 王文全 侯俊玲 周香珍 韩亚男 王丹)