UFLCQTRAPMSMS同时测定蚁巢伞属3种鸡中氨基酸核苷含量及PCA分析(1)
[摘要]建立高分离度快速液相色谱质谱联用(UFLCQTRAPMSMS)同时测定蚁巢伞属3种鸡中15种氨基酸和12种核苷类成分的方法。采用Waters XBridge Amide 色谱柱(21 mm×100 mm，35 μm)，流动相为02%甲酸水02%甲酸乙腈，梯度洗脱，柱温30 ℃，流速06 mL·min-1，采用电喷雾正离子源(ESI+)，多反应离子监测(MRM)模式进行检测。采用PCA主成分分析法对不同品种的蚁巢伞属鸡进行综合评价。结果15种氨基酸成分和12种核苷成分在一定浓度范围内均呈良好的线性关系，相关系数均大于0997 3，精密度，重复性和稳定性良好；加样回收率在9514%～1050%，RSD均小于50%。PCA分析显示在氨基酸中，鸡综合评价指数分最高；在核苷中，黄鸡综合评价指数最高。所建立的方法准确、重复性好，可用于蚁巢伞属3种鸡中15种氨基酸和12种核苷类成分的同时测定，为蚁巢伞属鸡资源内在质量的综合评价、全面控制以及合理开发应用提供新的方法参考。
[关键词]蚁巢伞属； 氨基酸； 核苷； 含量测定
, 百拇医药
[Abstract]A comprehensive analytical method based on UFLCQTRAPMSMS was developed for the simultaneous determination of 15 kinds of amino acids and 12 kinds of nucleosides of three species in Termitomyces The separation was carried out on a Waters XBridge Amide column (21 mm×100 mm，35 μm) with gradient elution of mobile phase of 02% formic acid in water02% formic acid in acetonitrile at a flow rate of 06 mL·min-1， and column temperature was maintained at 30 ℃ The target compounds were analyzed by the positive ion multiple reaction monitoring (MRM) mode The principal component analysis(PCA) was made to standardized treatment for the comprehensive evaluation of different species in Termitomyces The 15 kinds of amino acids and 12 kinds of nucleosides multiple constituents showed good linearity (r>0997 3) in the range of the tested concentrationThe average recoveries ranged from 9514% to 1050%，and the relative standard deviations were less than 50% The comprehensive evaluation index obtained with PCA showed that the Termitomyces albuminosus was significantly higher than others in amino acids and in nucleosides， of which the T aurantiacus was the best The developed method with good repeatability and accuracy was suitable for the simultaneous determination of multiple functional substances，which provided a new basis for the comprehensive assessment and overall control of the quality of Termitomyces fungi
, 百拇医药
[Key words]Termitomyces； amino acids； nucleosides； simultaneous determination
雞菌为担子菌亚门Basidiomycotina层菌纲Hymenomycetes伞菌目Agaricales离褶伞科Lyophyllaceae蚁巢伞属(原称鸡菌属)Termitomyces真菌鸡菌T. albuminosus (Berk) Heim的干燥子实体，是一类生于白蚁巢上的珍贵野生食药用菌。鸡菌属主要分布于非洲热带、亚洲热带、南太平洋岛屿和亚洲的亚热带地区，在我国主产于西南的云贵川，东南的福建和台湾等地，由于该菌与某些白蚁有互惠的共生关系，故其分布范围与大白蚁群Macrotermitinae的分布界限极为相近[17]。明代李时珍《本草纲目》[8]上就记载有“益胃，清神，治痔”的功效。现代研究表明，鸡菌具有抗氧化[9]、抗胃溃疡[10]、降血脂[11]、增强免疫功能[12]、镇痛抗炎[13]等药理作用。其不仅含有多糖、多酚等多种生物活性成分，还含有丰富的氨基酸、核苷等营养物质。
目前国内外对鸡菌功效物质研究主要集中于多糖、多酚等，核苷类成分尚未见报道，对于氨基酸的测定比较局限，大多需要经过衍生化处理，然而，大部分的衍生化法都具有衍生化试剂不稳定、操作繁琐、耗费时间以及产生副产物干扰测定等缺点[1415]。同时核苷类成分具有重要的生理功能，能够调节机体代谢功能，增强抗病能力，对肝脏、心血管及神经系统有重要的作用[16]。同时，近年来越来越多的研究证实人体内的某些氨基酸不仅作为蛋白质合成的底物原料，是人体生长发育必不可少的营养物质，也是神经递质、卟啉类化合物、聚胺类化合物合成的前体[17]。此外，氨基酸可用于分辨物质来源和保质期[1819]。, 百拇医药(张园娇 胡鹏 马赟 蔡静 李月 李祥 陈建伟)
[关键词]蚁巢伞属； 氨基酸； 核苷； 含量测定
, 百拇医药
[Abstract]A comprehensive analytical method based on UFLCQTRAPMSMS was developed for the simultaneous determination of 15 kinds of amino acids and 12 kinds of nucleosides of three species in Termitomyces The separation was carried out on a Waters XBridge Amide column (21 mm×100 mm，35 μm) with gradient elution of mobile phase of 02% formic acid in water02% formic acid in acetonitrile at a flow rate of 06 mL·min-1， and column temperature was maintained at 30 ℃ The target compounds were analyzed by the positive ion multiple reaction monitoring (MRM) mode The principal component analysis(PCA) was made to standardized treatment for the comprehensive evaluation of different species in Termitomyces The 15 kinds of amino acids and 12 kinds of nucleosides multiple constituents showed good linearity (r>0997 3) in the range of the tested concentrationThe average recoveries ranged from 9514% to 1050%，and the relative standard deviations were less than 50% The comprehensive evaluation index obtained with PCA showed that the Termitomyces albuminosus was significantly higher than others in amino acids and in nucleosides， of which the T aurantiacus was the best The developed method with good repeatability and accuracy was suitable for the simultaneous determination of multiple functional substances，which provided a new basis for the comprehensive assessment and overall control of the quality of Termitomyces fungi
, 百拇医药
[Key words]Termitomyces； amino acids； nucleosides； simultaneous determination
雞菌为担子菌亚门Basidiomycotina层菌纲Hymenomycetes伞菌目Agaricales离褶伞科Lyophyllaceae蚁巢伞属(原称鸡菌属)Termitomyces真菌鸡菌T. albuminosus (Berk) Heim的干燥子实体，是一类生于白蚁巢上的珍贵野生食药用菌。鸡菌属主要分布于非洲热带、亚洲热带、南太平洋岛屿和亚洲的亚热带地区，在我国主产于西南的云贵川，东南的福建和台湾等地，由于该菌与某些白蚁有互惠的共生关系，故其分布范围与大白蚁群Macrotermitinae的分布界限极为相近[17]。明代李时珍《本草纲目》[8]上就记载有“益胃，清神，治痔”的功效。现代研究表明，鸡菌具有抗氧化[9]、抗胃溃疡[10]、降血脂[11]、增强免疫功能[12]、镇痛抗炎[13]等药理作用。其不仅含有多糖、多酚等多种生物活性成分，还含有丰富的氨基酸、核苷等营养物质。
目前国内外对鸡菌功效物质研究主要集中于多糖、多酚等，核苷类成分尚未见报道，对于氨基酸的测定比较局限，大多需要经过衍生化处理，然而，大部分的衍生化法都具有衍生化试剂不稳定、操作繁琐、耗费时间以及产生副产物干扰测定等缺点[1415]。同时核苷类成分具有重要的生理功能，能够调节机体代谢功能，增强抗病能力，对肝脏、心血管及神经系统有重要的作用[16]。同时，近年来越来越多的研究证实人体内的某些氨基酸不仅作为蛋白质合成的底物原料，是人体生长发育必不可少的营养物质，也是神经递质、卟啉类化合物、聚胺类化合物合成的前体[17]。此外，氨基酸可用于分辨物质来源和保质期[1819]。, 百拇医药(张园娇 胡鹏 马赟 蔡静 李月 李祥 陈建伟)