大学生痤疮的病原学调查分析(1)
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[摘要]目的：分析大学生痤疮与螨虫、痤疮丙酸杆菌、金黄色葡萄球菌三种病原感染的相关性。方法：采集石河子大学中度、重度或重度集簇性颜面部痤疮患者样本55份，进行常规螨虫检查；根据痤疮丙酸杆菌和金黄色葡萄球菌16S rRNA序列，对采集样本细菌培养物进行PCR检测；用SPASS软件分析三种病原感染患者在性别、地域上的相关性。结果：螨虫阳性率为23.63%(13/55)，痤疮丙酸杆菌的阳性率为90.91%(50/55)，金黄色葡萄球菌阳性率为72.73%(40/55)。螨虫感染率高低与大学生来源地(疆内外)有统计学差异，但与性别无相关性；痤疮丙酸杆菌、金黄色葡萄球菌感染率高低与大学生来源地、性别无统计学差异。结论：痤疮丙酸杆菌、金黄色葡萄球菌、螨虫可能是中度、重度或重度集簇性痤疮患者的重要病原。
[关键词]痤疮；螨虫；痤疮丙酸杆菌；金黄色葡萄球菌
[中图分类号]R758.73+4[文献标识码]A[文章编号]1008-6455(2011)02-0272-03
Investigation on pathogenic analysis of college student acne
HOU Jian, LIU Chen-lan,HUANG Rong, WANG Jing, CHEN Ya-miao, LI Yong-xiang, WANG Yuan-zhi, LI Xiao-ju
(Medical College of Shihezi University,Shihezi 832002,Xinjiang，China)
Abstract: Objective To analyse the relation between college students'acne and infection of demodicid mite, prpropionibacterium acne and staphylococcus areus. Methods 55 samples of middle severe acne，severe acne or severe cluster-acne were obtained from face of Shihezi University students. demodicid mite were detected by conventional method. According to 16S rRNA sequence of P. acne and S. areus, two pair primers were devised. The optimal PCR condition were screened out and employed in 55 samples. Weather the difference existed between 3 pathogen infection and some factors, including sex, weather of born-land by analysis of SPSS software. Results The positive rate of D. mite, P. acne and S. areus were 23.63%, 90.91% and 72.73%, respectively. The significant difference existed between D. mite infection and born-land. Conclusion D. mite, P. acne and S. areus likely are important pathogens to the patients with middle severe acne, severe acne or severe cluster-acne.
Key words: acne; demodicid mite; propionibacterium acne; staphylococcus areus
痤疮是由毛囊及皮脂腺阻塞、发炎所引发的一种皮肤疾病[1]。根据引发的原因和发病机理不同可将其分为四级[2]：I级(轻度)：黑头粉刺散发或多发，炎症性皮疹散发；II级(中度)：I级+浅在性脓疱，炎症性皮疹数目增加，局限于面部；III级(重度)：II级+深在性炎症性皮疹，发生于颜面、颈部和胸背部；IV级(重度集簇性)：III级+囊肿，易形成瘢痕，发生于上半身。目前国外学者多认为痤疮不仅是皮肤病，更是一种与心理因素有着密切关系的疾病[3]，它使患者产生焦虑以及其他心理问题。在病原学研究方面，国内外学者一致认为痤疮的严重程度与螨虫、痤疮丙酸杆菌、金黄色葡萄球菌等[4-5]有关。本研究以石河子大学不同地域来源的大学生为研究对象，采集面部皮肤分泌物，应用镜检、细菌培养、PCR等研究方法，分析大学生痤疮的严重程度与哪些病原的入侵有关。该研究为痤疮的预防和治疗提供了科学依据，对痤疮的发病机制研究有一定借鉴意义 ......
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